Recovery and genotyping ancient Sicilian monumental olive trees
نویسندگان
چکیده
The long-lived and evergreen olive tree dominates the Mediterranean landscape, representing an agroecological cultural symbol a genetic heritage of inestimable value. Sicily, for historical, geographical, reasons, has very rich distinctive germplasm. In this work, large survey was conducted to discover, collect, characterize diversity centennial monumental trees from historical sites, such as Greek Temple Valley (Agrigento), ancient gardens, or farmland present in western part island. Trees were chosen based on their height, trunk, stump size, presumed age; particularly, only with age estimated at more than 400 years old taken into consideration. For morphological characterization, leaf, fruit, endocarp traits analyzed. molecular 11 polymorphic microsatellite markers largely used fingerprinting analysis used. Reference cultivars included comparison. Nuclear DNA extracted different parts plant (young leaves shoots canopy young suckers, which arose basal tree) check if grafted explore diversity. Most have been least one time during long life, some genotypes showed unique profiles combined peculiar phenotypic traits. Suckers (rootstock trees) strict relationship oleaster tree, also study. “Patriarch” (original mother plants) local identified. This research revealed high level still unexplored Sicilian germplasm highlighted its importance gene reservoir, could support new breeding programs evaluation possible selection linked putative resilience abiotic biotic stresses (particularly Xylella fastidiosa subsp. pauca ST53 soil- borne diseases insects). results will be useful improving conservation process, enriching existing collections resources, supporting on-farm projects.
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ژورنال
عنوان ژورنال: Frontiers in conservation science
سال: 2023
ISSN: ['2673-611X']
DOI: https://doi.org/10.3389/fcosc.2023.1206832